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IB Biology A3.1: Diversity of organisms worked solutions

Worked solutions to every practice question in A3.1: Diversity of organisms of Photon Biology: Study Guide for the IB Diploma (SL & HL). Try each question, then compare your working with the full solution.

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Basic practice

Question B1

[1 mark]
Hypothetical names: Vulpes vulpes, Vulpes lagopus, Alopex vulpes. Which inference follows? (A) The first and third are one species. (B) The first two share a genus. (C) All three have identical genomes. (D) The first two occupy the same habitat.
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B is supported because the first word assigns genus. A repeated second word across genera does not identify one species. Names alone establish neither habitat nor chromosome count.

Final answer

B. The first two share a genus.

Question B2

[2 marks]
A printer set a pea label as “pisum SATIVUM. Rewrite the scientific name using the usual print conventions, and identify its genus.
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Write Pisum sativum: initial capital for the genus, lowercase epithet and italics for both words [1]. The genus is Pisum [1].

Final answer

Pisum sativum; genus Pisum.

Question B3

[1 mark]
Under a reproductive definition of species, which result provides the strongest positive evidence that two populations belong together? (A) Adults share the same colour. (B) Pairs perform courtship. (C) Crosses produce fertile descendants. (D) Crossed offspring survive for a week.
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C tests successful reproduction in the next generation. Similar features, mating behaviour or offspring survival alone do not establish fertility.

Final answer

C. Crosses produce fertile descendants.

Question B4

[2 marks]
A cell contains seven homologous chromosome pairs. Each chromosome has two joined sister chromatids. Give the number of chromosomes and the number of chromatids.
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There are 7\times2=14 chromosome objects [1]. Replication supplies two chromatids per object, so there are 14\times2=28 chromatids [1]. Joined sisters are counted as one chromosome.

Final answer

14 chromosomes; 28 chromatids.

Question B5

[1 mark]
Two chromosome cut-outs have similar lengths. Which further comparison best tests whether they are homologues? (A) Centromere position and stain-band pattern. (B) Distance from the cell membrane. (C) Order in the photograph. (D) Age of the organism.
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A adds two structural matching criteria to length. Chromosomes need not be next to one another in the source micrograph; colour density alone is not a band-pattern comparison.

Final answer

A. Centromere position and stain-band pattern.

Question B6

[2 marks]
A human karyogram contains 44 autosomes, one X and one Y. Give its total chromosome number and the sex predicted by the supplied XX-female/XY-male model.
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Add all chromosome objects: 44+1+1=46 [1]. The X and Y provide an XY complement, predicting male under the supplied model [1]. This does not test every gene variant.

Final answer

46; male under the stated model.

Question B7

[1 mark]
For an ordinary green plant, which collection best represents its genome? (A) Only the genes currently expressed. (B) Every RNA molecule in the leaves. (C) All alleles in the local plant population. (D) Nuclear, mitochondrial and chloroplast DNA, with coding and non-coding regions.
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D includes all three DNA-containing compartments and does not restrict the definition to genes that are expressed. A population allele collection is a gene pool.

Final answer

D. Nuclear, mitochondrial and chloroplast DNA, with coding and non-coding regions.

Question B8

[2 marks]
An invented insect record gives a haploid nuclear size of 750 Mbp. Express it in Gbp and state whether this value alone establishes its gene number.
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750\,\mathrm{Mbp}/1000=0.750\,\mathrm{Gbp} [1]. DNA amount includes non-coding regions and does not count genes, so gene number cannot be deduced from this value [1].

Final answer

0.750 Gbp; gene number cannot be established.

Question B9

HL[1 mark]
Use this miniature key: 1a alternate leaves: A; 1b opposite leaves: go to 2; 2a smooth edge: C; 2b toothed edge: D. Identify a specimen with opposite leaves and a toothed edge.
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Opposite leaves send you from 1b to decision 2; the toothed edge then selects 2b, ending at D [1]. The decision sequence is an identification route, not an ancestry statement.

Final answer

D, by 1b then 2b.

Question B10

HL[2 marks]
A sequenced environmental barcode has no close match in the reference library. State one reason a detected organism could remain unidentified and one useful next step.
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A species may be absent from the reference set, so its read cannot be assigned reliably [1]. Add a reference from an independently identified specimen, or analyse an additional informative barcode region [1]. Do not invent a species identification from the best poor match.

Final answer

Its reference may be missing; expand the verified library or compare another informative region.

Tutorial practice

Question T1

[3 marks]
Two labels read Larus argentatus and Larus fuscus. (a) State the genus shared by the labels. [1] (b) Explain why a shared genus does not settle whether they form one biological species, and state the breeding result needed. [2]
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(a) The shared genus is Larus [1]. (b) The complete binomials differ, so the labels assign distinct species [1]. A biological test asks whether representative members can interbreed and produce fertile offspring; sharing a genus is insufficient [1].

Final answer

(a) Larus. (b) They are labelled as different species; breeding must produce fertile offspring to support one biological species.

Question T2

[4 marks]
Two island snail populations have distinct shell bands. Adults cross in captivity and the young survive, but the young have not yet bred. (a) Assess what the shell and cross observations establish. [2] (b) Propose the next breeding test, including a control. [2]
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(a) Different shell bands support a morphological distinction [1], but offspring survival demonstrates viability, not fertility, so a biological-species conclusion is premature [1]. (b) Rear hybrid offspring to reproductive maturity and test whether they produce offspring [1]. Use comparable within-population crosses as controls, with similar age and conditions, so a failure is not merely caused by the procedure [1].

Final answer

(a) Morphological grouping suggests two forms; viable young alone do not settle the biological criterion. (b) Test fertility with appropriate within-population controls.

Question T3

[4 marks]
The chromosome cut-outs have these illustrative features (length; fractional centromere position): a (1.4; 0.45), b (2.2; 0.65), c (1.8; 0.35), d (2.2; 0.65), e (1.4; 0.45), f (1.8; 0.35). Band positions also match within the identical feature groups. Each joined two-rod object is one replicated chromosome. (a) Give the three matching pairs using the letters. [1] (b) State two visible criteria supporting your pairing. [2] (c) State the chromosome total in this cell. [1]
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(a) Match a with e, b with d and c with f [1 for the complete pairing]. (b) Compare length, centromere position and staining-band pattern; award [2] for two precise matching criteria beyond simply calling them the same shape. (c) There are six chromosome objects [1]. Joined sister chromatids do not double this count.

Final answer

(a) a/e, b/d, c/f. (b) Length, centromere position and band pattern. (c) 6 chromosomes.

Question T4

[3 marks]
In a toy species, the usual diploid complement is eight chromosomes. Its arranged image is recorded as: group I, two chromosomes; group II, three; group III, two; sex group, X and Y. The supplied model associates XY with male. (a) Calculate the total. [1] (b) Identify the numerical change and its evidence. [1] (c) Deduce sex under the stated model. [1]
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The total is 2+3+2+2=9 [1]. Group II contains three rather than the usual two chromosomes, identifying the extra chromosome [1]. The sex group is XY, predicting male under the stated model [1]. No parental origin follows from this record.

Final answer

9 chromosomes; one extra in group II; male under the supplied model.

Question T5

[4 marks]
Synthetic database records give R: 1.8 Gbp per unreplicated diploid nucleus; S: 1200 Mbp per haploid nuclear set. (a) Give both haploid nuclear sizes in Gbp. [2] (b) Assess the claim that S must have more genes and be more complex. [2]
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(a) R has two unreplicated sets, so its haploid value is 1.8/2=0.9\,\mathrm{Gbp} [1]. S converts to 1200/1000=1.2\,\mathrm{Gbp} [1]. (b) A larger DNA amount need not mean more genes because non-coding sequence contributes [1]. Complexity needs a stated independent measure; these two records alone cannot rank it [1].

Final answer

(a) R: 0.9 Gbp; S: 1.2 Gbp. (b) No: size is not a gene count or a defined complexity measure.

Question T6

[4 marks]
An invented sequencing workflow takes eight days where its earlier version took 120 days, on the same genome and to the same accuracy. (a) Calculate the factor by which the time has fallen. [1] (b) State the molecular information the workflow determines. [1] (c) Explain one potential use that is specific to an individual patient. [2]
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(a) 120/8=15, so the time is reduced by a factor of 15 [1]. (b) Sequencing determines DNA base order [1]. (c) Identify variants in the individual patient [1], then use validated associations with drug response or risk to inform that patient care [1]. Sequence alone does not establish a variant function or prescribe treatment.

Final answer

(a) 15 times faster. (b) DNA base order. (c) Compare patient variants with established response evidence to inform an individual drug choice.

Question T7

HL[4 marks]
Species U has a normal diploid count of 16 and species V a count of 18. Assume ordinary haploid gametes fuse and no chromosome is lost. (a) Predict the hybrid chromosome number. [1] (b) Explain why fertility could be reduced. [2] (c) State why the chromosome total does not prove sterility. [1]
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(a) Gametes contain 16/2=8 and 18/2=9, so the hybrid has 8+9=17 chromosomes [1]. (b) Some parental chromosomes may lack matching homologues [1]; irregular meiotic segregation can then produce unbalanced gametes, lowering fertility [1]. (c) The count alone does not establish the actual pairing behaviour, so sterility is not certain [1].

Final answer

(a) 17. (b) Mismatched pairing can prevent balanced gametes. (c) Not certain from the count alone.

Question T8

HL[4 marks]
Four invented plants have these traits: W, alternate leaves and smooth edges; X, alternate leaves and toothed edges; Y, opposite leaves and smooth edges; Z, opposite leaves and toothed edges. (a) Construct a complete dichotomous key for these four plants. [3] (b) State how you would test it before field use. [1]
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One complete key is: 1a alternate leaves: go to 2; 1b opposite leaves: go to 3 [1]. 2a smooth: W; 2b toothed: X [1]. 3a smooth: Y; 3b toothed: Z [1]. Test each endpoint using every specimen, preferably with another observer using the key unaided, and revise any ambiguous decision [1]. Each alternative has a destination and the paired statements do not overlap.

Final answer

Split alternate/opposite, then smooth/toothed within each branch; endpoints W/X and Y/Z. Test all four routes independently.

Advanced practice

Question A1

[6 marks]
Researchers sample 30 adults from each of two valley moth populations. Wing patterns form two groups, but four measured body dimensions overlap. Ten attempted between-population crosses yield six broods. Fertility of these descendants is unknown. Within-population control crosses yield broods in nine of ten attempts. All numbers are invented. (a) Calculate the two brood-success percentages and their difference in percentage points. [2] (b) Evaluate whether these results establish two biological species. [2] (c) Design the next investigation that would most directly improve that judgement. [2]
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(a) Between-population success is 6/10\times100=60\%; controls give 9/10\times100=90\% [1]. Their difference is 90-60=30 percentage points [1]. (b) Distinct wing patterns support a morphological split [1], but overlapping dimensions and viable broods do not establish a complete reproductive barrier or descendant fertility [1]. (c) Rear descendants to maturity and test reproductive success [1], with comparable within-population controls, repeated crosses and matched conditions [1]. The small cross sample supports a provisional judgement rather than a firm species boundary.

Final answer

(a) Between: 60%; within: 90%; difference 30 percentage points. (b) Evidence is mixed and fertile descendants are not demonstrated. (c) Test descendant fertility with matched controls.

Question A2

[7 marks]
A synthetic size survey records fern F: 6.4 Gbp per unreplicated diploid nucleus; insect I: 800 Mbp per haploid nuclear set; animal M: 2.0 Gbp per haploid nuclear set. The survey also assigns independent structural-complexity scores F=4, I=7, M=9. (a) Standardise F and I to haploid Gbp. [2] (b) Calculate the F:I genome-size ratio. [1] (c) Evaluate a proposed universal increase of complexity with genome size. [2] (d) Explain what additional sequence information would help test evolutionary relationships. [2]
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(a) F contains two unreplicated sets: 6.4/2=3.2\,\mathrm{Gbp} [1]. I converts to 800/1000=0.8\,\mathrm{Gbp} [1]. (b) 3.2/0.8=4 [1]. (c) F has more DNA than I yet a lower score, providing a counterexample to an invariably increasing size-complexity claim [1]. Three chosen species and one scoring system cannot establish a universal relationship [1]. (d) Sequence corresponding regions in the taxa [1], then compare aligned differences across suitable regions to test relationship hypotheses [1]. Genome size itself is not an alignment.

Final answer

(a) F: 3.2 Gbp; I: 0.8 Gbp. (b) F/I=4. (c) The size ranking contradicts a universal increasing complexity rule; the sample and score are limited. (d) Compare homologous sequence regions, not size alone.

Question A3

[6 marks]
A hypothetical lineage changes from 12 chromosomes per haploid set to 11. Two separate ancestral chromosomes contain blocks J-K and L-M. One descendant chromosome contains J-K-L-M, an internal telomere-like tract at K/L and a second inactive centromere-like region. Treat these observations as invented evidence for evaluating a fusion hypothesis. (a) Give the ordinary diploid totals before and after the proposed event. [2] (b) Explain two observations supporting fusion rather than using the count alone. [2] (c) State an alternative explanation for a lower count and how the sequence observations help test it. [2]
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(a) Ordinary diploid totals are 2\times12=24 and 2\times11=22 [2, one each]. (b) Conserved blocks occur in the predicted joined order [1]. Internal end-like repeats or the inactive centromere provide an independent feature expected after joining two former chromosomes [1]. (c) Loss of a chromosome could also lower the number [1]. Mapping the retained ancestral sequence blocks in the descendant tests fusion against simple loss [1]. This is a testable reconstruction, not proof of the cause of a species split.

Final answer

(a) 24 and 22. (b) Conserved joined blocks, internal end-like DNA and an inactive centromere support fusion. (c) Count alone also allows chromosome loss; corresponding sequence retention helps distinguish it.

Question A4

HL[7 marks]
In an invented pond survey, a 200-base barcode from one sample matches bacterial reference strains R and S equally well. R and S differ across many other genome regions, but a resistance gene has recently moved from R into S. The same barcode is also detected in the sampling blank. (a) Explain two difficulties with applying the biological species concept here. [2] (b) Assess whether the barcode and shared gene establish one species. [2] (c) Interpret the blank and propose a stronger follow-up. [2] (d) State why read totals would not establish the number of living organisms. [1]
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(a) The strains reproduce without sexual crosses, so an interbreeding/fertility test is inapplicable [1]. Horizontal transfer moves a gene between groups outside parent-to-offspring inheritance, further weakening a simple reproductive boundary [1]. (b) Equal barcode matches leave the two references unresolved [1]. A transferred resistance gene does not establish whole-genome identity or one species, because the rest of the genome may have a different history [1]. (c) A positive blank is evidence of possible contamination [1]; collect independent samples with clean equipment and fresh blanks, and use additional informative regions or verified specimens [1]. (d) DNA amount or read number is affected by shedding, persistence and amplification, so it cannot directly count living individuals [1].

Final answer

(a) Asexual reproduction prevents a fertility test; gene transfer can cross group boundaries. (b) The barcode cannot distinguish R/S and a shared transferred gene does not merge whole lineages. (c) The blank raises contamination; repeat clean, independent samples and compare independent regions. (d) Reads do not count living organisms.

Connected questions

Question C1

Connected questions · HL[6 marks]
A3.1 + D2.1 + A4.1. In an invented sexual species, one individual has two non-homologous ancestral chromosomes joined into one fused chromosome, retaining the same sequence blocks. Its diploid total is 23; most members have 24. Do not assume that all meiotic products are balanced. (a) Give the usual haploid number and explain why the carrier count need not mean less DNA. [2] (b) Explain a possible meiotic problem in the carrier. [2] (c) State the evidence needed before concluding that the fusion caused a separate species. [2]
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(a) The normal haploid number is 24/2=12 [1]. Joining two chromosomes into one reduces the count by one without requiring loss of their sequence blocks [1]. (b) The fused chromosome must associate with the corresponding regions on two unfused partners [1], so segregation can create unbalanced chromosome combinations and reduce the production of functional gametes [1]. (c) Test actual fertility or offspring chromosome balance rather than declaring inevitable sterility [1]. Evidence that gene exchange is persistently restricted is needed before attributing a species boundary to the rearrangement [1].

Final answer

(a) The normal haploid count is 12; fusion can reduce count without proportional DNA loss. (b) Homologous regions may pair in a complex arrangement, affecting segregation and balanced gamete formation. (c) Fertility and reproductive isolation need direct evidence.

Question C2

Connected questions[6 marks]
A3.1 + A3.2 + D1.3. Two named populations have identical haploid genome sizes of 1.4 Gbp. A same-direction alignment of 500 comparable bases contains four differences. The sites lie outside the barcode used in an earlier survey. All values are invented. (a) Calculate the percentage difference in the segment. [1] (b) Explain two limits on the conclusion that these are identical genomes. [2] (c) State what further sampling would establish whether the variable positions are common polymorphisms. [2] (d) Suggest an additional sequence comparison that could test ancestry. [1]
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(a) 4/500\times100=0.8\% of the aligned positions differ [1]. (b) Equal size states DNA amount, not base order [1]. A 500-base fragment is not a representative whole-genome estimate by itself [1]. (c) Sample multiple individuals from each population [1] and measure alternative-base frequencies at the aligned sites to determine whether the variants are common within either population [1]. (d) Compare additional corresponding regions with reliable references or an outgroup to test evolutionary relationship hypotheses [1]; no species boundary follows from the four differences alone.

Final answer

(a) 0.8%. (b) Equal amounts do not establish sequence identity; the segment cannot establish whole-genome divergence. (c) Sample more individuals to assess common polymorphism. (d) Compare additional corresponding regions to test relationships.

Question C3

Connected questions · HL[6 marks]
A3.1 + A4.2 + C4.1. A conservation survey detects a barcode assigned to a rare aquatic plant in two water samples. Sampling blanks are negative. The site is downstream of a population known to contain that plant. A local key has separate endpoints for the rare plant and a similar common species. (a) Give two biological explanations for the detection. [2] (b) Design a follow-up combining environmental sampling with the local key. [2] (c) State one requirement that makes the key distinguish the two plants reliably. [1] (d) Explain why these detections do not estimate population size. [1]
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(a) The rare plant may occur locally [1], but DNA transported from the upstream population could also explain the result [1]. (b) Take replicated samples along the watercourse and look for local specimens [1]; identify them using verified diagnostic traits and the tested key, providing independent morphological evidence [1]. (c) Use paired non-overlapping statements about the same feature and test the route on both known species [1]. (d) A detection does not estimate abundance because DNA concentration depends on shedding, transport, decay and laboratory amplification, not just organism number [1].

Final answer

(a) DNA may be local or transported downstream. (b) Use replicate upstream/downstream samples and identify observed plants with a verified key. (c) Make diagnostic decisions exclusive and test both species. (d) Detection does not estimate abundance.

From the book

Photon Biology: Study Guide for the IB Diploma (SL & HL)

Diversity of organisms from the core ideas to full exam questions, with question types, worked examples and graded practice.

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